Stamceller i tarmscancer präglade av uttrycket bmi1 eller lgr5 bidrar

Farmakologiskt blockerar p53-beroende apoptos skyddar

Young (2–3-month-old) and old (> 19-month-old) Lgr5-EGFP-IRES-creERT2 mice (The Jackson Laboratory) were total-body irradiated with or without 10 Gy of X-rays and sacrificed 8 h later. Swiss roll sections of paraffin-embedded intestines were prepared and immunohistochemically analyzed. May 8, 2014 GFP knockin allele: Yy1f/f;Vil-creERT2; Lgr5-EGFP-IRES-. creERT2 (43).

Matrigel Mar 29, 2012 immunostaining in Lgr5-EGFP-IRES-CreERT2 and wild-type mice. We compared Lrig1 and Lgr5 expression, as Lgr5 is an established SC Apr 25, 2012 Mice lacking TLR4 in Lgr5-positive intestinal stem cells were gener- ated by first crossing the Lgr5-EGFP-IRES-creERT2 mice (12) with mT/mG. Jun 21, 2012 using the knock-in allele Lgr5EGFP-Ires-CreERT2, we Distribution of stem cells marked by Lgr5-GFP and Paneth cells, marked by lysozyme Lgr5陽性細胞でGFPとCreリコンビナーゼタンパク質を発現するLgr5-EGFP-Ires- CreERT2トランスジェニックマウスを、通称レインボーマウスと呼ばれる Sep 20, 2012 We visualized Lgr5-EGFP+ve cells in neonate Lgr5-. EGFP-ires-CreERT2 kidney sections using anti-GFP immunohis- tochemistry (IHC) (Figure To measure the overlap of Krt19+ cells with Lgr5+ cells, we performed Krt19 in situ hybridization on colonic (Figure 2A) tissues of Lgr5-EGFP-IRES-CreERT2 Methods Studies were performed in Lgr5−EGFP-IRES-creERT2 , Bmi1-CreERT2 , Hopx-CreERT2 , and TRE-H2BGFP::Hopx-CreERT2::lox-stop-lox-tdTomato determined the effects of the chemoprotective FPA diet on miRNAs and mRNAs in colonic stem cells obtained from Lgr5-EGFP-IRES-creERT2 knock-in mice.

Lgr5 - H Rob Ky

To induce the expression of the reporter gene, a single We crossed Lgr5-EGFP-IRES-CreERT2 knock-in and ROSA26-tdTomato (LRT) mice, and treated them with or without 4-hydroxytamoxifen (4-OHT), to determine Aug 1, 2012 To study the behavior of Lgr5+ cells within Apc-mutant adenomas, we then crossed Lgr5EGFP-Ires-CreERT2/Apcfl/fl mice with the R26R-Confetti Exogenous IFN-γ depletes Paneth cells and modifies the expression of rapidly cycling ISC markers. Heterozygous Lgr5 Egfp Ires Creert2 Mice, supplied by The Jan 15, 2021 Organoids from Lgr5-EGFP-ires-CreERT2-TdT mice were transplanted in C57Bl6 male mice exposed to PIR. Engraftment and repopulation of (C) MGs were isolated from Lgr5-EGFP mice and analyzed by flow cytometry for iments, Lgr5-EGFP-IRES-creERT2 mice were crossed into the LifeAct-RFP. Also Known As:Lgr5-EGFP-IRES-creERT2.

Snabbt cyklande lgr5 + stamceller är utsökt känsliga för

These two mouse strains were crossed to generate the Lgr5 EGFP‐Ires‐CreERT2; Rosa26‐LacZ mice. All animals were housed in a facility with a 12‐h light/12‐h dark cycle. ApcMin and Lgr5-EGFP-ires-CreERT2 mice were obtained from The Jackson Laboratory (Bar Harbor, ME). Foxf1 and Foxf2 targeted mutants have been described elsewhere.22,23 Tg(FOXF2) was generated by pronuclear injection of a linearized BAC (RP4-668J24) spanning 120,429 base pairs of the human FOXF2 locus. tiation. We have employed the crypts from Lgr5‐EGFP‐IRES‐creERT2 reporter mouse line to grow Lgr5+ enteroids.10 In this article, we sum‐ marize the procedure of organoid culture derived from single Lgr5+ cells, and provide a simple way to examine their viability and functions.

Gene: Lgr5-eGFP-IRES-CreERT2 mice, where GFP- positive Lgr5+ cells resided at the base of the glands in both the corpus and the antrum, GFP-expressing Lgr5+ cells were hardly observed in -Cre; Cdh1fl/fl; SPA Lgr5-eGFP-IRES-CreERT2 mice (Figure 1C).
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1 A–F and N). To examine the relative contribution of Lgr5 vs. Bmi1 ISCs to tissue regeneration under steady-state conditions Lgr5-EGFP-Ires-CreERT2 and RosaSOR-LacZ mice (Jackson Laboratories, Bar Harbor, USA) were crossed to incorporate the LacZ reporter for lineage tracing upon administering tamoxifen . These Lgr5-EGFP-Ires-CreERT2/R26R-LacZ mice were also backcrossed into a hairless background using Crl:SKH1-HR hairless mice (Charles River, Sulzfeld, Germany). stochastically initiated using the Lgr5-EGFP-Ires-CreERT2 allele in conjunction with the R26R-Confetti reporter. Analysis of stem cell clones was performed at various time points after Cre-activation by tamoxifen in 10-week-old mice, after which the progeny of these Lgr5hi cells were mapped in 3D-reconstructed crypts. H pylori colonize and manipulate the progenitor and stem cell compartments, which alters turnover kinetics and glandular hyperplasia.

2007-10-14 · Figure 4: EGFP expression in an Lgr5-EGFP-IRES-creERT2 knock-in mouse faithfully reproduces the Lgr5-lacZ expression pattern in the intestinal tract. 2018-01-09 · Lgr5-EGFP-Ires-CreERT2;R26R-Confetti double transgenic mice were treated with vehicle (n = 8) or G007-LK (n = 5) and tamoxifen according to the two administration schemes outlined in Fig. 1d, f. In control mice, lineage traced cells populated the entire length of the villi (Fig. 2 a) in the majority (69.9%) of the villi (Additional file 3 : Figure S2A). 实验动物商城平台.
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Lgr5-EGFP-IRES-creert2 (Lgr5-EGFP) möss tillhandahölls av Dr Eduard Batlle vid Institutet för forskning i biomedicin i Barcelona, Spanien. Experiment Expression of LGR5, an Intestinal Stem Cell Marker, During Lgr5+ve Stem Cells Drive Self-Renewal in the Stomach and . Lgr5 gene · Lgr5 antibody · Lgr5+ intestinal stem cells · Lgr5-egfp-ires-creert2 · Lgr5 wnt · Lgr5620kq1 · Lgr5 protein Lgr5-EGFP-IRES- creERT2 knock-in-möss matades dieter innehållande n-6 PUFA (kontroll), n-3 PUFA, n-6 PUFA + curcumin eller n-3 PUFA + Lgr5-EGFP-IRES-CreERT2; Rosa26-floxed-tdTomato-möss användes för att generera nya data för uppskattning av klonala expansionsparametrar i pylorkörtlar Vi har alla Lgr5 Samling av foton. Lgr5. Lgr5 Gene Lgr5 Antibody. Hemsida.

Experiment Expression of LGR5, an Intestinal Stem Cell Marker, During Lgr5+ve Stem Cells Drive Self-Renewal in the Stomach and .
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Arts medierar apoptos och regenerering av intestinal stamcells nisch

Mice were maintained on a 12-h:12-h light/dark schedule in a temperature-controlled speciﬁc pathogen-free facility and fed stan-dard laboratory mouse chow. The Lgr5 EGFP-Ires-CreERT2 knock-in mice (Barker et al., 2007) and Rosa26-LacZ reporter mice were obtained from The Jackson Laboratory. To induce Cre recombinase in Lgr5 EGFP-Ires-CreERT2 ; Rosa26-LacZ mice , tamoxifen (Sigma) was dissolved in corn oil and injected intraperitoneally from postnatal day 3 to 4 (P3–P4; 0.3 mg/g body weight). Young (2–3-month-old) and old (> 19-month-old) Lgr5-EGFP-IRES-creERT2 mice (The Jackson Laboratory) were total-body irradiated with or without 10 Gy of X-rays and sacrificed 8 h later. Swiss roll sections of paraffin-embedded intestines were prepared and immunohistochemically analyzed. May 8, 2014 GFP knockin allele: Yy1f/f;Vil-creERT2; Lgr5-EGFP-IRES-.

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To induce the expression of the reporter gene, a single Mus musculus, C57BL/6J, ET993, 3 to 9 (month), young, female, Lgr5-EGFP- IRES-creERT2, epithelium of small intestine, intestinal crypt stem cell, young 1 Aug 2012 To study the behavior of Lgr5+ cells within Apc-mutant adenomas, we then crossed Lgr5EGFP-Ires-CreERT2/Apcfl/fl mice with the R26R-Confetti 21 Jun 2012 Retracing of Lgr5+ adenoma cells. (A) Apc-deficient adenomas were allowed to develop for 24 days in Lgr5-EGFP-Ires-CreERT2/Apcfl/fl/R26R Also Known As:Lgr5-EGFP-IRES-creERT2. Heterozygous mice harbor an Lgr5- EGFP-IRES-creERT2 "knock-in" allele that both abolishes Lgr5 gene function were generated by crossing Lgr5–EGFP–ires–CreERT2 and. Klf5 flox/flox mice ( 4, 29). Because Cre–ERT2 fusion gene activity is inducible following tamoxifen In 4 Lgr5-EGFP-IRES-CreERT2 Kras LSL-G12D mice studied between 2 and 8 months after tamoxifen.

Kvantifiera lgr5-positivt stamcellsbeteende i pylorepiteln

The Lgr5 EGFP-Ires-CreERT2 knock-in mice (Barker et al., 2007) and Rosa26-LacZ reporter mice were obtained from The Jackson Laboratory. To induce Cre recombinase in Lgr5 EGFP-Ires-CreERT2 ; Rosa26-LacZ mice , tamoxifen (Sigma) was dissolved in corn oil and injected intraperitoneally from postnatal day 3 to 4 (P3–P4; 0.3 mg/g body weight). Long-term culture of adult progenitor cells in 3D is a recently emerging technology that inhabits the space between 2D cell lines and organ slice culture.

Lgr5-EGFP-ires-creERT2 mice were crossed with APCﬂ/ﬂ mice. Cre enzyme activity was induced by intraperitoneal injections of tamoxifen (2 … 2007-11-01 Intestinal cell fractions from Lgr5-EGFP-ires-CreERT2 mice, expressing GFP under the control of the Lgr5 promoter. RNA was isolated from two FACS sorted cell populations: stem cells were sorted based on high level of GFP expression (GFPhi) and Paneth cells were sorted based on high level of CD24 expression (CD24hi) and high side-scatter (SSChi).